Phosphatidylinositol kinase activity associates with viral p60src protein.

Phosphatidylinositol 3-Kinase Activation Syk Associates with Clathrin and Mediates

Hepatitis C virus NS5A protein interacts with phosphatidylinositol 4-kinase type IIIalpha and regulates viral propagation.

Hepatitis C Virus (HCV) nonstructural 5A (NS5A) is a pleiotropic protein involved in viral RNA replication and modulation of the cellular physiology in HCV-infected cells. To elucidate the mechanisms of the HCV life cycle, we identified cellular factors interacting with the NS5A protein in HCV-infected cells. Huh7.5 cells were electroporated with HCV Jc1 RNA. Cellular factors associated with HC...

Hepatitis C Virus NS5A Protein Interacts with Phosphatidylinositol 4-kinase Type III and Regulates Viral Propagation

Hepatitis C Virus NS5A Protein Interacts with Phosphatidylinositol 4-kinase Type III and Regulates Viral Propagation Yun-Sook Lim and Soon B. Hwang* From the National Research Laboratory of Hepatitis C Virus, Ilsong Institute of Life Science, Hallym University, Anyang, 431-060, Korea Running head: HCV NS5A modulates PI4KIII * Address correspondence to: Soon B. Hwang, Ph.D. Ilsong Institute o...

Characterization of avian and viral p60src proteins expressed in yeast.

Avian and viral p60src proteins were expressed from a galactose-inducible promoter in the yeast Saccharomyces cerevisiae. Both the viral and cellular src proteins produced in yeast cells were myristoylated at their amino termini, as is the case for src proteins expressed in chicken embryo fibroblasts. The viral src protein produced in yeast autophosphorylated at tyrosine-416 in vivo and had app...

Phosphatidylinositol 3-kinase c2alpha contains a nuclear localization sequence and associates with nuclear speckles.

Phosphoinositide 3-kinase C2alpha (PI3K-C2alpha) belongs to the class II phosphatidylinositol 3-kinases, which are defined by their in vitro usage of phosphatidylinositol and phosphatidylinositol 4-phosphate as substrates. All type II phosphatidylinositol 3-kinases contain at their C terminus a C2-like domain. Here we demonstrate that Homo sapiens phosphoinositide 3-kinase C2alpha (HsPI3K-C2alp...